DNA

Part:BBa_K2053000:Design

Designed by: Mathieu Hubert   Group: iGEM16_Pasteur_Paris   (2016-10-11)


T. pseudonana silaffin kinase (tpSTK1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1322


Design Notes

Polyhistidine tag (6 His) was added at the N-terminal


Source

This part comes from Thalassiosira pseudonana and we took the sequence by using its GenBank accession number (EED92887) [Sheppard V et al, J Biol Chem. 2010 Jan 8;285(2):1166-76, doi: 10.1074/jbc.M109.039529, Epub 2009 Nov 4].

References